FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379155
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2301.094053E-625.24261
TCCTAGG2351.3195167E-624.7055232
GTACTAG5400.022.8468881
CTAGGAC3503.5033736E-922.7861653
ACCTAGG1600.002162968322.6788981
CTAGACA4607.2759576E-1222.0656534
TAGGTAG3955.8935257E-1022.0257515
CTAAGAC4951.8189894E-1221.970133
TAGGACT4358.367351E-1121.6670974
AGGACCG3259.833384E-720.077325
GTATTAG7300.019.8828681
AGGGCGC2205.905578E-419.77248
AGCCTAG4051.8619176E-819.7110441
TACTCCG3351.314902E-619.4779975
GGGTAGG4904.0927262E-1019.253921
ACCGTGT3401.5159094E-619.1908598
GTCCCGA1900.005855119.0980191
TAGACAG5759.094947E-1218.9134185
CTATCCT8100.018.7966674
GACTGTG4253.2750904E-818.7643977
Produced by FastQC (version 0.11.5)