FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234826
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG19350.032.9729775
GTATTAG35000.027.9945641
TAGTCAG19650.026.932769
GTATAGA40350.024.6425151
CTAATAC40350.023.8981673
GGTAGTC24100.023.7646067
TATTAGG36250.022.624472
CTATGCA33700.022.1578669
CTAGACT15450.022.0559564
CCTAATA38900.021.456372
TAATACT62750.021.1443234
TATACCG23350.020.8038835
TATACAG47550.020.5844735
ACTATGC39550.020.163548
CTATACT39950.019.96334
GTCCTAG11750.019.7660581
GACTAGG14350.019.7030418
TACTATG42900.019.6029437
TCTATAC40750.019.2155423
CTTAGAC23500.019.1284713
Produced by FastQC (version 0.11.5)