FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890547
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG17000.025.1620145
TATACGG24950.021.503225
GTATTAG31050.019.4046651
TATACAG43450.018.6883345
ACGGTGT23100.018.2029428
TAGTCAG19000.017.5521289
TAGGACA18650.017.1047294
GTATAGG24600.015.9348621
GTATAGA35900.015.7721121
GGTAGTC22550.015.7534287
ATACGGT28900.015.5531886
TACACAG23400.015.4916065
TACACCG14700.015.2892675
TCTATAC38100.015.22324853
GTATATA83550.015.1179351
CTATACT34700.015.0433774
TTAGGAC17000.014.9266193
CTAATAC35600.014.8667213
TATACCG21150.014.7400995
ACTATGC31500.014.7297368
Produced by FastQC (version 0.11.5)