FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379445
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG5300.023.2541719
ACCTCGC2303.1437077E-522.06478
GTATTAG9200.021.2975181
GTATAAG9750.019.3518181
ACCCGAG1900.005889795319.0784997
GGACCGT2400.001060155118.1245756
CAGCGTG3602.6187336E-618.1245739
GAGCGAG2000.00791863318.1245737
GTACTAG6152.5465852E-1117.6998351
TAGGTAG4959.749783E-917.5778945
CGGGGCT2952.1268491E-417.2029869
GTATATG13400.016.7884241
TATAAGA12650.016.6364462
GTGTAGG4057.964927E-616.1265161
GTCTTAC5854.327376E-916.1265161
TACTCGG2700.002332356516.1107337
GTATAAT13150.016.0038811
GGTAGTC6459.404175E-1015.7360647
GTATTAA12550.015.6125241
ATAAGAG11250.015.4685463
Produced by FastQC (version 0.11.5)