FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490934
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC3203.389323E-822.6556788
TACACGG2857.0930564E-620.3537775
TAGGTAG6151.8189894E-1218.8644755
TAGGGGT3101.4657644E-518.7123434
CCGTGCA3151.6847964E-518.4122359
TATACGG7600.018.1275845
AACCGTG4003.567402E-718.1245447
TATACAG12650.017.7693315
TATACCG6603.6379788E-1217.5782625
GTATAAG10850.017.3914971
GTACTAG7750.016.8563751
CAGCGTG3905.6169592E-616.7303479
CTAATAC10950.016.5548693
ATACAGC10100.016.5094856
ATTATAC18050.016.4704913
GTATTAG9750.016.3761371
GCCCGGC4007.145525E-616.312099
TATTATA26750.016.2787422
GGGGCCC4901.6642662E-716.2750996
ACAGCGT7151.4551915E-1116.2233668
Produced by FastQC (version 0.11.5)