FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7838359
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG55750.020.9641321
GTATTAG51050.019.4814261
GTATAGA52700.019.1469731
TATAAGA71150.017.4468542
TATACCG37150.017.3696885
TATACAG61500.016.5049345
TAGGTAG28000.016.3133585
CTAATAC59150.016.057483
GTATATA101700.015.9890611
TAATACT92300.015.5533644
TATATAC112050.015.3354793
GTATTAT96300.015.2272331
TATTAGG54100.015.162722
GTATAAT76500.014.89821151
ATAAGAG67350.014.8560153
TATAATA113650.014.8188482
ACCGTGC17850.014.2150748
CCTAATA55050.013.5823782
GTATAGG41900.013.3405151
GTGTTAG46600.013.2412471
Produced by FastQC (version 0.11.5)