FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30472597
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG202050.021.5545941
GTATAAG228350.021.4878581
GTATAGA222800.020.5896681
TAGGTAG119100.019.0541765
TATAAGA292400.017.351912
TAATACT354500.016.545884
GTATATA369650.016.0820221
TATTAGG211350.016.0727832
CTAATAC237600.015.7151433
TATACAG231950.015.6290725
GTATTAT377300.015.6020421
CCTAATA211350.015.3859112
GTATAGG171700.015.0074011
GTATATG253850.014.8973151
ACTATGC230150.014.8680878
TATACTG293200.014.4907635
CTATGCA179950.014.1812619
GACTAGG100550.014.1318198
ATAAGAG270500.014.0986023
GTACTAG146300.013.9414981
Produced by FastQC (version 0.11.5)