FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618555
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG1153.1444925E-431.5494042
CGGAGCA950.00472620130.5259429
GTATTAG4800.030.2348481
GTATAAG5000.029.0254541
CGGCCCC1100.00966936426.3633149
GTGCAGG2808.107236E-925.9155831
GTATAGA5350.025.7702621
TATTAGG4850.023.9385192
TCCAGGG3052.0127118E-823.7913532
CAGCGCA2151.8644218E-523.6043629
TACACCG1851.8297191E-423.5132265
TATAAGA7200.022.1722222
AGACTGC2653.7764603E-621.8865246
GTAGACT2653.7764603E-621.8865244
ACTATGC4659.094947E-1221.827698
GTGTAAG3004.5108573E-721.769091
ACACCGT3055.3340045E-721.3931796
ACAGCGC1700.003091696421.3232678
CTAATAC5101.8189894E-1221.3232673
GACTAGG1700.003091696421.3232678
Produced by FastQC (version 0.11.5)