FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466066
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13950.020.7897325
CTAATAC34050.020.2287753
GTATTAG25150.019.3400651
CTATGCA23850.017.9344949
CCTAATA31900.017.751092
CTATACA27800.017.4740334
GTATAGA29450.016.762731
ACTATGC29000.016.4995258
CCTACAC11450.016.4638333
TACACAG21200.016.4160425
TAGACTG15950.016.3645845
GTCTAGG15350.016.0802081
GTACTAG17300.015.946251
TATACAG33300.015.8943115
ACCGTGC8250.015.8177278
TCGGTCG7352.1827873E-1115.781869
TAGACCG20900.015.610955
TAGTCAG14500.015.4995559
CCTATAC22700.015.3312833
CTAGGAC9500.015.264043
Produced by FastQC (version 0.11.5)