FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4565546
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG21300.022.4646195
GTATTAG37400.021.7346321
TAGTCAG21550.015.81169
TATACAG53200.015.2630425
TACACCG17050.014.88258655
GTATATA97800.014.8421561
GTATAGA39750.014.7895091
TATACTG56550.014.7434785
TATTAGG48450.014.3808092
ATAGTAC38200.013.8546083
GTGTAAG22750.013.7180671
GTATATG55700.013.6816971
GGTAGTC27250.013.5684087
TATACGG31050.013.0755855
TATTATA130650.013.0546222
CTAGGAC12850.012.9765563
TAATACT70600.012.9389954
GTCTACA28800.012.852381
CTAATAC38900.012.6734273
CATATAC58000.012.6249063
Produced by FastQC (version 0.11.5)