FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645091
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG450.00963861548.3320437
GACGTGC1352.1414218E-532.2213636
GAGGTCC1204.0534168E-430.212214
TAGACCG2405.259062E-827.1909895
AGGACCG1409.983255E-425.8961815
AGGACAG2259.088926E-725.7810865
TAGACAG3108.56744E-1025.7291095
TCTAGAC2951.4239049E-824.5794243
GTAGGAC1851.8280956E-423.5165313
CTAGACA3402.4956535E-923.458894
TACACCG1902.1900922E-422.8976755
TGACGTG2252.6470316E-522.558455
AACCGTC1952.6138223E-422.3070957
ACCGTGT1650.002600271521.969118
CCGGCCC2003.0986965E-421.7527924
GCACCGT2003.1019465E-421.749426
TAGAAGT5350.021.6850244
TAGTACC4358.367351E-1121.6694474
TCTAGGA2404.3036955E-521.1813872
AGAAGTG5850.021.0710815
Produced by FastQC (version 0.11.5)