FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7215988
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG45050.022.7213671
GTATAGA47300.019.4918351
ACCGTGC15300.018.0057878
CTATGCA40150.017.6954049
TAATACT81400.017.6367244
CTAATAC53700.017.552793
GTATAGG37250.017.5398711
ACTATGC47400.017.4359848
TATACAG53150.017.3251725
TATACCG30350.017.200895
TACACAG32600.016.9033625
GTACTAG30100.016.882691
TATACTG71200.016.5991085
TAGACCG30800.016.0079355
CCTAATA49200.015.640512
GTATAAT71300.015.0689281
CTAGACT22150.015.0577894
GTATATA81850.014.9891891
TTATACT64400.014.9741754
GACCGTC29100.014.9478317
Produced by FastQC (version 0.11.5)