FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4316983
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG36800.023.273241
TAGGTAG20950.021.4566655
CTAATAC41800.019.9469343
GTATAGA41150.018.520051
TATTAGG42900.018.272132
TAGACCG24750.018.162315
TATACTG55950.018.0123045
CTATGCA35600.017.9207889
GACTAGG14900.017.516238
GGTAGTC25250.017.5143037
CCTACAC13750.017.4006633
GTCTAGA20950.017.3224241
TATACAG51250.017.2591955
TAGTCAG22350.017.1918569
TACACAG27150.017.090895
GTATATA97600.017.0297531
CCTAGGA9000.016.9355552
TATACGG26600.016.8991415
CCTAATA44400.016.8374732
CTAGGAC12500.016.8206423
Produced by FastQC (version 0.11.5)