FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557122
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11200.026.5709781
TAGGTAG6250.025.523365
ACTCCGC4700.023.1376278
CTACCCC3501.998018E-618.645314
GACGCGC2359.2050084E-418.5101018
TATACGG7301.8189894E-1216.8857825
GGTAGTC7800.016.7302847
TCTAGGG5652.759407E-916.683613
CCGGCCC3052.7436236E-416.638869
ATAGGAC6556.548362E-1116.6052383
ACCGCGC2650.002058855516.4146188
GTCCTAA6751.0186341E-1016.1298071
GTATAGG10400.016.052261
CTAATAC13650.015.9361633
TCTATAC13650.015.9361633
AGTGTGC4551.3733861E-615.9336058
GTACTAG8200.015.9331041
TAATACT20850.015.6495414
GTGTATA13000.015.6335061
ATACTAT20000.015.2245586
Produced by FastQC (version 0.11.5)