FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674179
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6550.023.2458695
GTATTAG12600.023.0401631
GTATAAG12800.022.680161
GTATAGA15700.022.1889971
CCGATCG2053.6683862E-421.2190679
GTCTTAG7150.020.301121
GACTAGG5002.5465852E-1120.2995748
CCTAGGA4202.8258E-819.0081332
GTATAGG10250.018.4096531
TAGTCAG6750.018.2588759
TTAGGTA12400.018.1262444
CTAATAC13650.018.0598473
TATACTG19800.017.2107775
GACCGAC2952.1271515E-417.2030287
TATTAGG14100.016.985992
GTATATA27000.016.9345191
CTATGCA12550.016.7526369
TATAGAG16500.016.7145882
ACTAGGC6556.548362E-1116.6027039
CTACACA8800.016.4784014
Produced by FastQC (version 0.11.5)