FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313708
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG7850.023.1091061
TAGGGCG1600.002175192822.6568184
GTATAGA7500.022.2525271
GTGTAAG3304.6500645E-821.9886651
GTGTGCA4751.2732926E-1121.3681459
TAACCCG2053.6666627E-421.2200475
GTCCTAG2404.3352902E-521.164091
GACCGCG2455.1160783E-520.714027
CAGGCCG1750.00365960520.714029
TTAGACG4652.055458E-1020.2693274
GTACAGA5103.274181E-1119.9191421
CTAATAC8750.019.8862153
TATACGG5153.8198777E-1119.7092345
TGTAAGG5254.9112714E-1119.3500252
CTCTAGG3001.0977499E-519.3500231
TAGACAG5657.2759576E-1219.2482725
GTATTAT12500.019.1565231
CCGAGCG1900.00588928719.0787039
CTACACT6500.018.9620154
GTATTAG6900.018.929371
Produced by FastQC (version 0.11.5)