FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397956
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG11000.036.9542161
TATAAGA14600.026.847852
TAGGTAG5550.026.1262555
ATAAGAG13250.025.717113
CTATACT9650.023.2902724
GTATTAG9850.021.3712711
CTAGGAC3208.4752355E-720.3907263
GACAGTG8550.020.350257
ACCTCGC1900.00589008219.078368
TAGTCAG5851.0913936E-1118.589179
TAGGACA5551.10958354E-1018.2883784
GGTAGTC6401.8189894E-1218.1244417
TATTAGG10900.017.980672
CGTACCG2450.001217213517.755195
CACCCCG2050.00912979217.682387
GTATAGA10800.017.4750421
TAAGAGA18100.017.2238434
TTATACT13950.017.1506234
GTATAGG8500.017.0796781
GTCTAGG6404.5474735E-1117.012961
Produced by FastQC (version 0.11.5)