FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2925380
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG11800.023.347795
GTATTAG21300.022.8285081
GTATAAG22800.022.2815571
CTAATAC24550.021.263033
TACACCG9800.020.7145795
CCACGCG3208.483785E-720.3902179
GTATAGA25650.019.2399481
TATACTG30300.018.6636315
CCTAATA23350.018.648622
TATAAGA30850.018.5846442
TAGACCG17650.017.663145
TAATACT37500.017.5935824
GACTAGG9550.017.4603828
CTATGCA20100.017.3130869
ACACCGT16600.017.0327916
ATAAGAG27450.016.90373
TCGGTCG6907.2759576E-1216.8112589
GTCCAGA18700.016.300091
GTCTAGG14250.016.2973671
GGTAGTC14250.016.2803767
Produced by FastQC (version 0.11.5)