FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691620
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG700.001056541841.427569
CCCTAAG1501.1238608E-633.8692361
TAGGTAG2309.986252E-1031.5300875
GTATTAG5100.025.6153891
TAGGACA3251.4788384E-924.544964
TATACCG3251.4788384E-924.544965
ACTACAC3702.582965E-1023.5197413
TACACCG1851.8265897E-423.5197415
CTTAAGG1952.6089334E-422.3136023
ACCGTGT2053.6654677E-421.2189928
GATTAGG1750.003635193720.7362672
CACCGTG1750.003658123820.713787
GTAGGAC1800.004299156420.1442223
ACCCCGT1800.004306360620.1383956
GTATTAC6250.019.7409271
GGCCCTA1850.00504604519.5941186
ACTGAGC2608.074235E-519.5187538
AGAGTCC3001.1049497E-519.332868
ATCGTGC2659.346123E-519.1504768
GAGAGTC3151.6826587E-518.4122497
Produced by FastQC (version 0.11.5)