FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168116
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCC502.0070632E-457.9903646
CCGGCCC400.0060461454.3659678
GACTGAC400.0060461454.3659679
GGCCTAT450.00960899548.3540651
CGTGGAC604.930073E-448.3396843
TCCGGCC450.0096316248.3253027
GGCGGTA450.0096316248.3253028
AGGCTTG803.7249796E-545.3049747
GGTTACC750.001476756338.6832541
AACCCTT750.001481083638.660246
CTGTACA800.002030496536.254764
CTTATCC1001.3854592E-436.254763
CTAAAGT800.002030496536.254764
ACGAGGC800.002033465336.2439778
TCTTAAG850.002729781734.1322822
GAATCCT900.00361738732.2264565
CGTAAAC900.00361738732.2264563
TTGTATG1153.1485746E-431.5258774
CTTCAGG1000.00604759129.012441
CATTACA1255.122955E-429.012442
Produced by FastQC (version 0.11.5)