FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31094
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGG100.007058044144.999981
CGCTGGT100.007058044144.999987
TATAGAT301.5750571E-596.6666642
ATTGATC300.00191806472.58
AGATGTC300.00191806472.55
ATAGATG406.562113E-572.499993
TAATACT350.00353405262.1428574
GTATATA350.00353405262.1428571
ATATAGA604.870929E-448.3333321
ATAGTAA450.00955207948.3333321
GGGGGGG89900.09.435483145
AAGGGGG5101.0970325E-88.529411145
AGGGGGG5102.0372681E-107.107843135-139
AAAAAGG2200.0099598776.5909085130-134
AAAAGGG5601.1383236E-86.214286130-134
AAAGGGG5258.625084E-75.8130-134
Produced by FastQC (version 0.11.5)