FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377278
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19500.028.2915531
TAGGTAG9900.027.829495
GACTAGG6800.025.587738
GTATAGA19850.025.2328591
ACTATGC17950.023.4257228
TAGACCG14000.022.7867035
GTCTACA19950.022.1954921
TATACCG11500.022.0661745
GTCTAGG10550.022.0178741
TAGTCAG10950.021.8488859
CAGTGCG5350.021.6818149
CTAATAC19350.021.3575153
TAATACT28550.021.3319244
TTAGGTA18050.021.2890114
TACACCG8650.020.9546665
CTATGCA15250.020.917629
GGACCGT4901.8189894E-1120.7138776
TACTATG18550.019.9322227
TAGACAG15100.019.6862855
CCTAGAC10400.019.5200773
Produced by FastQC (version 0.11.5)