FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326728
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6200.036.2501345
GTATTAG11000.032.333351
CTAGACT5150.030.9709874
CCTACAC4100.030.0610873
TACACCG5100.028.4314755
CAGCGTG5500.026.3617469
TAGTCAG7700.026.3617469
CTATGCA10100.024.404199
CCTAATA11600.024.4036032
CCTCGCG1500.001495154324.1649349
CTAATAC12900.023.6047383
CCGTCGC6150.023.5755449
GTATAGA11700.023.574641
TAGACCG8950.023.4917075
ACCTCGC2852.7821443E-722.8930958
TAATACT16600.022.7109264
TACAGCG6800.022.38817
TACTATG11700.022.3060917
GGTAGTC9150.022.18427
ACTATGC13400.022.1812448
Produced by FastQC (version 0.11.5)