FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512840
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG12450.030.3044871
GTATTAG9800.025.1724681
ATAAGAG13300.023.9860133
TATAAGA16050.023.507222
GGACCGC1550.001809150923.3865746
TAGGTAG5250.022.0962055
TGGACCG3757.767085E-921.26765
CTAGGAC4551.5279511E-1020.7151933
GACCGCT3208.478419E-720.390177
TAGTCAG6100.020.2044669
TATAGTG11150.019.507585
GTATAAT15650.019.471881
GAGACAG11850.018.354027
GTGTAAG6351.8189894E-1218.281811
TAAGAGA19400.017.9389294
GACCGTC6503.6379788E-1217.8457557
TCTAGGA7600.017.1843012
CAGGACG4659.156793E-817.1512894
AGACCGT8900.017.106366
GGACCGT3403.2481883E-517.0584436
Produced by FastQC (version 0.11.5)