FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180426
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG18950.027.1856171
GTATTAG16650.022.6610071
TATAAGA23700.021.7370242
TAGGTAG7800.021.3783725
CTTACAC8750.018.2287143
TATACCG9300.017.9302465
GTAGGAC6102.1827873E-1117.8280073
ATAAGAG20650.017.5546153
TATACTG21550.017.4943355
GAGACAG17500.017.3993367
TAGGGGT5451.713488E-917.293714
ACCTCGC4205.953643E-717.2612488
TAAGAGA27750.017.2433764
GTGTAGG6755.456968E-1217.1991231
CTGTGCG3804.3888613E-617.1703999
GTATAAT21750.017.0137881
TAATACT28200.016.9682164
GTATAGA19150.016.2925761
TATTAGG16550.016.2216052
CTAGGGG3605.3009295E-516.1112373
Produced by FastQC (version 0.11.5)