FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587924
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG13950.022.8837831
TATAAGA17850.022.3549562
TAGGACA7550.021.1263334
GTATAGA12900.019.122231
ACCGCGC3101.468158E-518.7089468
GTACTAG7400.018.6282161
CTAATAC12600.018.4131373
GTATAGG9900.018.321211
TAATACT19200.018.1254334
GTGTAGG4908.538336E-917.7678361
TAGCACG3352.8593491E-517.3138474
CCTAATA11100.016.9941622
CCCTAAG3052.7280982E-416.6512781
ATAAGAC10950.016.5529063
TATACAG13350.016.2925245
CCTAGAC6256.020855E-1016.2403893
CTATAAG8050.016.2228071
ATAAGAG14400.016.1114963
GTATATA23950.016.0553471
CTATGCA10100.015.7914639
Produced by FastQC (version 0.11.5)