FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429430
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18350.022.9390451
TAGGTAG10550.020.6181555
TACACCG7750.019.6471045
ACCGTGT7750.019.6446788
CTTACAC9750.019.3352473
TATACCG11300.017.966385
TATAATA35900.017.385512
CCTAATA17850.017.0763282
TATACAG20450.017.0187995
CTAGGAC6857.2759576E-1216.9359823
CTAATAC19300.016.9058173
GTATAGG14350.016.6895831
GTATAGA19600.016.2922121
ACTATGC16750.016.0145328
CCTATAC13900.015.6490313
TATAGGG11200.015.5516562
CCGTGCG2800.00297353615.5353339
TATACTG24500.015.3892785
GTATTAA22200.015.364851
TAATACT30650.015.140164
Produced by FastQC (version 0.11.5)