FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_C01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_C01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3988531
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30900.024.1940121
TAGGTAG16800.023.7363575
GGTAGTC18150.021.5694797
CTAATAC37250.020.4373073
TAGTCAG16000.020.38999
ACTGCGC9250.018.810148
CCTAATA33900.018.6272492
TATACTG42050.018.1043935
ACCGCGC6503.6379788E-1217.8455188
CTAGTAC20850.017.0392573
CTATGCA26100.016.943849
GTATAGG24500.016.8864351
GTATAGA31750.016.4595491
TATACAG38900.016.4018825
CTAGGAC11500.016.3921593
ACTATGC35900.016.3573578
TAATACT56400.016.3262064
TATTAGG31700.016.2565442
TAGACAG24100.016.245665
TATACCG20350.015.6764945
Produced by FastQC (version 0.11.5)