FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039488
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG37050.017.8296091
TAGGTAG19650.016.9733075
TATACAG50100.016.787715
TATACTG61800.016.777175
GTATAGA38600.016.5494651
GTATATA92950.016.2443581
TACACCG16500.015.3799615
TATACGG29600.015.186975
CATATAC56700.014.5778333
GTATTAA55100.014.36031
GTATAAG40950.014.3588521
TCTATAC40100.014.103323
GTATAAT60650.014.0037291
TATAATA110200.013.899192
TATACCG25650.013.8509615
GACTAGG11900.013.4027888
GTATTAT80300.013.3793431
TTATACT61900.013.2360674
CCTAATA45700.013.1841012
TATATAG58850.013.1828265
Produced by FastQC (version 0.11.5)