FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294056
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC27750.022.7307533
GTCCTAG8250.021.9988651
ACTATGC24750.021.6762288
GTACTAG16700.020.8659881
TATACAG24350.020.2473165
CTATGCA20700.018.9125739
GTATAGA22200.018.6395781
GTATTAG23700.018.3787981
GACCGTC15800.018.3539737
GTCCTAA15450.018.3252681
CTAGTAC17200.018.1258163
CTAGACT12000.018.1258144
TAGACAG18800.018.1258145
AGACCGT21650.018.0826916
TACTATG25600.017.5581577
TATAGTG21100.017.5244855
CTATACT28250.017.4521124
TAGACCG19900.017.1238865
CTAGACA17750.016.747234
TAATACT43300.016.5769584
Produced by FastQC (version 0.11.5)