FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081070
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC4303.6379788E-1223.6362551
CCCGGGT1700.003094487421.3228388
TATACAG14650.020.7863065
CACCCCG3751.8111314E-719.3327067
TCCTTGC11900.018.885948
CTTCCTA8100.018.821461
CCTTGCG7050.018.5100369
GTATCCT10300.018.3254741
CAGCGCA6401.8189894E-1218.1244139
CTATACT11850.017.7437464
ATCCTAT8850.017.2264211
CCTTAAG7650.017.0816632
GTGTAAG6455.0931703E-1116.883041
ACTCCGC7301.8189894E-1216.8830138
TCCTACA8650.016.785452
TAGACCG8650.016.7640725
GTATAGA10900.016.6507031
TAGACAG9750.016.3600165
TATACGG7651.8189894E-1216.1121375
CTCCTAC10050.015.8918941
Produced by FastQC (version 0.11.5)