FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6824632
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG30800.025.1877945
GTATTAG51900.024.8981441
TAGTCAG32200.020.038239
TATTAGG59600.019.4889342
CTAATAC62150.019.1319753
ACTATGC54700.019.0853638
GTATAGA57400.018.8446771
CTATACA54150.018.4772664
CCGTGCG6700.018.3949839
CTATGCA44850.018.265919
TAGACAG40000.018.1257975
GGTAGTC36400.017.9252997
TCTATAC58250.017.6745973
GTGTAGG21850.017.6091651
TATACTG80350.017.4152035
TACTATG60550.017.3611767
TATACAG68550.017.3457665
TACACCG26550.016.3849015
CCTAATA62550.016.3646262
TTAGGTA56150.016.2696384
Produced by FastQC (version 0.11.5)