FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5931257
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT18500.025.4744764
CTGTGCG10700.025.0690369
CTATGCA33850.022.2738049
CCGTGCG6350.021.6929
TAGACCG28150.021.6353825
ACTATGC40400.021.174858
ACCGTGC13100.021.0296488
TATACAG47400.020.8029165
CTATACT41600.020.5661184
GTCCTAG14150.020.5194381
ACCGTCG26800.020.2883268
GTCTAGT24000.020.2640131
TAGGTAG21900.020.1952555
GTATTAG41600.020.0662571
CTAGGAC17350.020.058823
TATACTG60300.019.5995
GTATAGA45150.019.453131
TAATACT72450.019.4145344
TCTATAC48600.019.2449633
GTCTAGG23400.019.2325881
Produced by FastQC (version 0.11.5)