FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925036
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG1952.604902E-729.746255
ACTCCGC2951.4551915E-1129.4909768
GCCTAGG1306.4383296E-427.9172951
TACACCG2654.5529305E-927.3609395
TAGGTAG4300.026.9791565
GTCCTAC1907.0898623E-626.7418291
ACCATGC3607.2759576E-1226.1800698
CCTAGGA1701.0230342E-425.6182232
GTATTAG7650.025.6182211
CTCCGCA3751.0913936E-1125.1328669
GGTAGTC3801.2732926E-1124.802177
TATACGG4800.024.1688275
GTATAGA9050.024.0613141
TAGTCAG5000.023.1995689
ACTGTGC3854.274625E-1022.596988
ACAGTGC4006.89397E-1021.7495948
TCCTACG2353.675431E-521.6210562
ACCGTCG3901.2194505E-820.4483388
CCATGCG3551.01472324E-720.4221559
GTACTAG5052.7284841E-1120.1225641
Produced by FastQC (version 0.11.5)