FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218252
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG9200.028.4002821
TAGGTAG4200.027.6205795
ATAAGAG10800.024.839343
GACTAGG3854.274625E-1022.5968028
GTATTAG8650.021.8155021
GTCCAGG3706.5720087E-921.577391
GTCTTAC4851.6370905E-1120.9504951
TATAAGA12350.019.9811252
CACCGTG3651.3611316E-719.8624847
GTATAGA9450.019.200661
TAGGACA6900.018.9140934
GGGGGGC2307.9647294E-418.9125398
GTATTAC9000.018.547841
GTATTAT15550.018.2029671
TAAGAGA15150.018.185834
GTCCTAA5601.2369128E-1018.1446251
TACACCG4404.866706E-818.1260075
TAGAGCG2400.001059496818.1260055
GTCCTAG3252.183199E-517.8654771
ACCACGC3252.2041158E-517.845688
Produced by FastQC (version 0.11.5)