FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891819
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG16150.030.1087421
TATAAGA20650.026.3591582
ATAAGAG17550.023.9616243
GAGACAG13000.023.4226977
GTATAGA15450.022.5477431
CTACACA8550.021.2001694
GTCCCGG2755.1678926E-621.1128861
GTATTAG13750.020.0572431
TACACCG7450.019.4643155
GTAGGAC4954.7475623E-1019.0416033
TAGGACA8850.018.8429974
TATACCG9250.018.8119965
GTCCTAA7750.018.7291741
TATACAG16800.018.557725
TAGACCG8600.018.5476825
CCGCGTA2000.0079193618.1247069
TTATACT16500.018.0162894
CTAATAC13900.017.734933
GTACTAG9850.017.6833821
TCTAGAC8650.017.6022663
Produced by FastQC (version 0.11.5)