FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991980
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13600.025.091551
GTATAAG16700.022.1728881
GTATATA28550.020.0904451
CCTACAC6550.019.9255663
GTCTAGG8500.019.6461521
GTATTAT28100.019.378651
GTACTAG10450.018.0644871
TAGGTAG8050.018.0141415
TATAAGA20450.017.041842
CTAATAC15000.016.918283
TAATACT24650.016.7663044
TATACGG9250.016.461035
GTATAGA16350.016.4305671
TATTAGG14850.016.134522
TACACAG10300.015.4869145
CTAGGAC5654.982394E-815.3996993
TATAGTG12800.015.2944275
TATACAG17100.015.2646155
CAGGGGA5705.560105E-815.2626989
GTGTTAG11950.015.1894031
Produced by FastQC (version 0.11.5)