FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_B02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_B02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844591
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA15850.022.897491
GTCCTAA8500.022.2024841
CTAATAC15750.020.2557353
CTATGCA12300.020.0402289
GACTAGG5505.456968E-1219.772318
ACCGTGC4452.6102498E-919.5501488
ACTATGC15700.019.3944938
CCACGCG2256.8716676E-419.3329249
GGGGGGC3451.7439907E-618.9126438
GTGTATA12400.018.146261
GTATAGG12000.018.146261
CCTAATA16100.018.033552
CTACACA8850.018.0241724
GCCCCGC2450.001217626917.7547266
TAATACT23350.017.6996174
TACTATG16550.017.5222877
GTATATA28300.017.440931
ACTAGGC6705.456968E-1217.3130669
TATACCG10100.017.2292275
TATTAGG14000.017.1093312
Produced by FastQC (version 0.11.5)