FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4006905
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG33200.020.9901681
CTAATAC37000.018.8127983
TAGGCCG5801.0913936E-1118.7519785
GTATAGA33750.018.0670931
CTATGCA30050.017.8530129
CTGTGCG10150.017.1423119
ACCGTGT11700.016.7302388
ACTATGC32500.016.2840988
TAGGTAG18850.016.1555525
TATACAG39950.016.1531435
TAATACT54550.015.6845154
ACCGCGC6551.1714292E-915.4956918
ATACTAT54600.015.4024416
GTATAGG26000.015.07659151
TATTAGG35150.015.0757972
GTATATA75550.014.9890071
CCTAATA37550.014.8855052
TCTATAC34250.014.6073813
GGTAGTC19550.014.4624567
TAGAGCG10650.014.2972835
Produced by FastQC (version 0.11.5)