FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298378
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13500.031.6854935
GTATTAG25050.026.658531
TAGTCAG15350.023.1424969
GGTAGTC18300.022.1857
TAGACCG19550.020.025775
TAGGACA16900.019.3049224
ACTATGC27000.019.0641338
TATTAGG27900.018.732012
TAATACT46500.018.7098584
CTAGGAC9750.018.5899263
GGAGCGC3902.7372698E-718.589088
AGACCGT21100.018.5538396
CTAATAC29200.018.3734663
AGTGTGC12950.017.9144178
TATACGG16600.017.90685
ATACTAT42150.017.8878546
GGGAGCG4908.627467E-917.7544677
CTTACAC13950.017.6704243
TCTATAC27550.017.631753
TATACCG16050.017.6169935
Produced by FastQC (version 0.11.5)