FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751074
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG12650.021.7812715
TATACGG17750.020.0164895
TACACCG9900.019.7751035
CTATACT24250.019.1363184
GTATAGA25150.019.0513321
GTCCTAA11850.018.9916521
GTATAAG22600.018.9523471
GTATTAG21400.018.658141
GGGGGGC5601.2551027E-1018.1245428
TATACTG35650.018.1017535
CCGCGTG2450.001217867217.7546529
CTTACAC13100.017.712053
ATACCGT16900.017.5883126
TAGGTAG11200.017.4797785
GTCTAAG14650.017.3440231
ACCGCGC3804.3891723E-617.1706188
CTAATAC25850.017.1103733
ACTATGC24500.016.866928
CTATGCA18400.016.1544829
AGTGTGC9600.015.85897358
Produced by FastQC (version 0.11.5)