FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4769472
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG37200.024.1899931
TAGGTAG20800.023.3545865
GTATAGA41050.021.7444761
CCTAATA41900.021.1301612
TATACTG56650.020.8616375
CTATGCA32700.020.839889
CTAATAC42300.020.7398553
TAGTCAG22000.018.78319
ACCTAAT45300.018.5830421
TATTAGG42200.017.5406112
GGTAGTC24550.017.4227097
TCTATAC40800.017.4151253
ACTATGC40050.017.3773618
TAATACT65600.017.3522784
TAGACAG31150.017.2240125
TAGACCG25700.017.209075
TATACCG25050.017.076745
TACTATG42750.016.788597
GTATATA83250.016.736861
CTATACT40800.016.5265984
Produced by FastQC (version 0.11.5)