FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825952
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13250.024.0762065
GTATAGA28050.023.8060861
TAGACCG20800.023.354085
TACACCG12900.023.043345
TAGACAG22600.021.8148275
ACTATGC31050.021.7140668
GTATTAG25900.021.2983951
CTAATAC32800.020.557033
AGACCGT25700.020.0283436
CCTAATA26950.019.9299412
GTCCTAG10250.019.8274671
CTATGCA22400.019.7424769
GTACTAG20250.018.996951
TAATACT42850.018.7812044
CCTAGAC18950.018.7472743
CTAGACA20500.018.3908064
ACTCCGC14000.018.124248
CAGCGTG13250.018.0558479
ACTGCGC9650.018.030338
ACCCTGC16250.017.8454048
Produced by FastQC (version 0.11.5)