FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958194
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC3151.035005E-925.3168498
GGTAGTC8900.024.4376137
GTCCTAG4800.024.1901741
TAGGTAG7850.023.0897945
TATTAGG15800.022.9653532
GGACCGT4154.5474735E-1122.7102956
TAGTCAG8200.022.1031259
GTATAGA14550.021.9457261
TATACGG10250.021.2200835
GACCGCG2755.2159667E-621.0903997
TATACAG17750.020.4230845
GTATTAT27150.020.3143981
TAGACCG8950.020.2519425
GTATTAG15400.019.791961
CCCGGGG2205.9070933E-419.7722517
TACACTG13500.019.3338555
GTGTAGG6051.8189894E-1219.1922041
TACACCG6051.8189894E-1219.174075
GTACTAG9850.019.155671
CTATACT15200.018.6024744