FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677962
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG11450.032.3368231
ATAAGAG13750.026.8924453
GTATTAG10600.026.7110771
GTATAGG9250.023.5457291
GGGGCGT1851.8317877E-423.5127546
GTACTAG8100.023.3034921
TAGGTAG6250.023.2013265
CCGTGCA4154.5474735E-1122.710119
CTAGTAC8350.022.5761413
TATAAGA16450.022.5082
GTATAGA11000.021.1198061
TATACCG7400.020.57555
GAGACAG12150.020.2874137
TAGGACA7650.019.9030974
GTTATAC10000.019.5761183
TAATACT20200.019.3822964
CTAGACT4202.858178E-818.989184
TTAGGTA9550.018.980144
CAGTGCG3101.4681529E-518.7090729
CTAGACC9850.018.4020674
Produced by FastQC (version 0.11.5)