FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774596
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC12750.029.0030483
GACTAGG4251.8189894E-1223.88158
TATACAG12450.023.2956225
AGTGTCC5950.023.1504348
TAGGGGT3955.8935257E-1022.027634
CCTAGGC2353.708256E-521.5980173
GACCGCG3055.34681E-721.3926777
ACCGTGC3457.5155185E-821.0137428
GTCCTAG4151.0786607E-920.9861661
ACTAGGC4901.8189894E-1120.7135459
GTATAGA13000.020.6565761
GGAGTGT8200.020.3346396
GTATAAG10400.020.2378621
CCGTGCC2556.963279E-519.9012499
TCTGGAC10950.019.86513
TAGGGCG2205.9016567E-419.7748055
TAATACT18000.019.7381884
TCCTAGG3001.096204E-519.353912
CCTAATA10200.019.2116012
TACTATG11750.019.1269767
Produced by FastQC (version 0.11.5)