FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116788
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG1301.6552478E-533.460673
GATCGCG1050.00770980727.618337
CTAGGAC3255.0931703E-1126.7685383
TACACCG3108.58563E-1025.7251385
TCTATAC9300.024.9455913
CTATGCA6700.023.8053539
CAGCGTG3854.274625E-1022.5968179
CTAATAC9450.022.24813
AGTGTGC3304.692447E-821.9691288
GTAGACC4751.2732926E-1121.3678674
TCCTAGG1700.003073451621.3468722
TATACAG8550.021.198285
GTCCTAA4801.2732926E-1121.168981
GGGTGCC2755.212736E-621.0903637
CTATACA8950.021.0609074
CCTAATA8700.020.8561382
CCCCTAG1750.003635871520.736961
GTCCAGA7100.020.444891
ATACTAT13550.020.3315756
GTATTAT13250.020.2674451
Produced by FastQC (version 0.11.5)