FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_A01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_A01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882333
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA28450.023.4698981
TAGGTAG15800.022.9443865
GGGGGGC8950.021.0610628
GTATTAG28550.020.0829091
GTCCTAA18450.020.062241
CTAATAC34500.019.7547843
TAATACT49450.019.647264
CCTAATA28650.019.5061592
GTCCTAG10050.018.7764261
GTCCCGG4303.7032805E-818.5664921
CCTACAC12600.018.4137783
GTGTAAG16600.018.3631341
TATTAGG28100.018.0799562
TAGGACC16150.017.957714
CTATGCA23900.017.8971589
GTCCAGG11450.017.7483581
TACACAG17400.017.5010265
CTAGGAC12550.017.3316963
CCGCACG10250.016.9752949
TATACTG36800.016.7469085
Produced by FastQC (version 0.11.5)