FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4949417
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG48150.016.7145165
TAGGTAG18550.016.4161955
GTATATA82150.016.1720051
GTATTAG37950.016.0689641
TATATAC93550.015.0357483
CTAATAC41500.014.8503923
GTGTATA31950.014.76941
TATAATA96250.014.5571682
ACTATGC40450.014.33805758
ATACAGG35800.014.1753526
CTATACT43150.013.9464744
GTATAGA40650.013.9301041
TATACGG27000.013.6953635
TATTATA102600.013.4439412
TAATACT69100.013.4307134
GTATTAT75200.013.4189121
TATACTG54100.013.1339675
CTAGGAC12900.012.9272233
GTATATG49250.012.6768861
GTATAAT56300.012.6368261
Produced by FastQC (version 0.11.5)