FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4336609
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGA24850.027.423787
TATAGGT32750.023.2463043
AGGTGCG30600.022.7444616
ATACAGG24300.022.6742636
TATACAG31250.021.1138445
GATATAG43750.020.5764681
TAGGTGC35550.019.987685
GTGCGAC35200.019.5662148
ATAGGTG38000.019.4622274
TACAGGC23900.019.1103427
ACCTCGC9300.018.7091548
CCTCGCG8350.018.2330239
TGCGACG39000.017.8456559
GTATTAG25200.017.2853371
TAATACT48050.017.2023354
ATACTAT31200.017.195036
GACCTCG9400.016.967617
GTATAGA32600.016.9247841
TATATAC55350.016.7674983
CTAATAC28550.016.7615343
Produced by FastQC (version 0.11.5)